Taking Simultaneous Snapshots of Intrinsically Disordered Proteins in Action.

ABSTRACT: Intrinsically disordered proteins (IDPs) as well as intrinsically disordered regions (IDRs) of complex protein machineries have recently been recognized as key players in many cellular functions. NMR represents a unique tool to access atomic resolution structural and dynamic information on highly flexible IDPs/IDRs. Improvements in instrumental sensitivity made heteronuclear direct detection possible for biomolecular NMR applications. The CON experiment has become one of the most useful NMR experiments to get a snapshot of an IDP/IDR in conditions approaching physiological ones. The availability of NMR spectrometers equipped with multiple receivers now enables the acquisition of several experiments simultaneously instead of one after the other. Here, we propose several variants of the CON

SUBMITTER: Schiavina M 

PROVIDER: S-EPMC6626832 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature