Improving the Repeatability and Efficacy of Intradermal Electroporated Self-Replicating mRNA.

ABSTRACT: Local administration of naked self-replicating mRNA (sr-mRNA) in the skin or muscle using electroporation is effective but hampered by low repeatability. In this manuscript, we demonstrated that intradermal electroporation of sr-mRNA in combination with a protein-based RNase inhibitor increased the expression efficiency, success rate, and repeatability of the data. The RNase inhibitor should be added just before administration because storage of the inhibitor together with the sr-mRNA at -80°C resulted in a partial loss of the beneficial effect. Furthermore, the location of intradermal electroporation also had a major effect on the expression of the sr-mRNA, with the highest and longest expression observed at the tail base of the mice. In contrast with previous work, we did not observe a b

SUBMITTER: Huysmans H 

PROVIDER: S-EPMC6626868 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature