Hemogenic Endothelial Cells Can Transition to Hematopoietic Stem Cells through a B-1 Lymphocyte-Biased State during Maturation in the Mouse Embryo.

ABSTRACT: Precursors of hematopoietic stem cells (pre-HSCs) have been identified as intermediate precursors during the maturation process from hemogenic endothelial cells to HSCs in the aorta-gonad-mesonephros (AGM) region of the mouse embryo at embryonic day 10.5. Although pre-HSCs acquire an efficient adult-repopulating ability after ex vivo co-culture, their native hematopoietic capacity remains unknown. Here, we employed direct transplantation assays of CD45^-VE-cadherin(VC)⁺KIT⁺(V⁺K⁺) cells (containing pre-HSCs) into immunodeficient neonatal mice that permit engraftment of embryonic hematopoietic precursors. We found that freshly isolated V⁺K⁺ cells exhibited significantly greater B-1 lymphocyte-biased repopulating capa

SUBMITTER: Kobayashi M 

PROVIDER: S-EPMC6626887 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature