Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality.

ABSTRACT: In vitro surrogate models of human cardiac tissue hold great promise in disease modeling, cardiotoxicity testing, and future applications in regenerative medicine. However, the generation of engineered human cardiac constructs with tissue-like functionality is currently thwarted by difficulties in achieving efficient maturation at the cellular and/or tissular level. Here, we report on the design and implementation of a platform for the production of engineered cardiac macrotissues from human pluripotent stem cells (PSCs), which we term "CardioSlice." PSC-derived cardiomyocytes, together with human fibroblasts, are seeded into large 3D porous scaffolds and cultured using a parallelized perfusion bioreactor with custom-made culture chambers. Continuous electrical stimulation for 2 weeks prom

SUBMITTER: Valls-Margarit M 

PROVIDER: S-EPMC6626888 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature