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Screening miRNAs associated with resistance gemcitabine from exosomes in A549 lung cancer cells.

ABSTRACT:

Purpose

To establish a gemcitabine-resistant lung adenocarcinoma cell line, A549/G+, and to screen the differences of miRNA expression in exosomes from A549 and A549/G+ cells.

Methods

A549 cells were exposed in gemcitabine until they were resistant to gemcitabine, and extracted exosomes from A549 and A549/G+. The RNAs from exosomes were subjected to miRNA expression microarray experiments.

Results

After 39 weeks of continuous induction, we induced drug resistance in A549 cells. The resistance index was 6. Via GeneChip miRNA 4.0 analysis, there were 446 differential miRNAs between A549 and A549/G+. Target gene prediction and pathway analysis discovered the microRNAs in the intersections may participate in drug resistance.

Conclusion

These differential miRNAs help to do in-depth research to elucidate the mechanism of resistance to gemcitabine in non-small cell lung cancer.

SUBMITTER: Liang AL

PROVIDER: S-EPMC6626902 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Screening miRNAs associated with resistance gemcitabine from exosomes in A549 lung cancer cells.

Liang Ai-Ling AL Du Shen-Lin SL Zhang Bin B Zhang Jing J Ma Xuan X Wu Cui-Yun CY Liu Yong-Jun YJ

Cancer management and research 20190708

<h4>Purpose</h4>To establish a gemcitabine-resistant lung adenocarcinoma cell line, A549/G+, and to screen the differences of miRNA expression in exosomes from A549 and A549/G+ cells.<h4>Methods</h4>A549 cells were exposed in gemcitabine until they were resistant to gemcitabine, and extracted exosomes from A549 and A549/G+. The RNAs from exosomes were subjected to miRNA expression microarray experiments.<h4>Results</h4>After 39 weeks of continuous induction, we induced drug resistance in A549 ce ...[more]

PMID: 31372037

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