Kbtbd11 gene expression in adipose tissue increases in response to feeding and affects adipocyte differentiation.

ABSTRACT:

Aims/introduction

The putative tumor suppressor gene, KBTBD11, might play a role in tumorigenesis, and is associated with cellular apoptosis and proliferation in colorectal cancer cells. However, the function of Kbtbd11 during adipogenesis is unknown. The aim of the present study was to investigate the role of Kbtbd11 in the differentiation of 3T3-L1 preadipocytes.

Materials and methods

For the fasting-refeeding protocol, mice were subjected to fasting for 24 h, followed by a chow diet for 12 h. Adenovirus infection methods were used to examine the effect of Kbtbd11, and 3T3-L1 cells were analyzed with Oil Red O staining and real-time polymerase chain reaction.

Results

The white adipose tissue expression of Kbtbd11 messenger ribonucleic acid (mRNA) was significantly h

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC6626956 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature