Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.

ABSTRACT: Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria. The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment. The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype. Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment. Alpha-diversity decreased upon inoculation of the ex vivo fermentation under all treatments. Co

SUBMITTER: Asto E 

PROVIDER: S-EPMC6627432 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature