Pharmacological Properties of the Type 1 Tyramine Receptor in the Diamondback Moth, Plutella xylostella.

ABSTRACT: Tyramine receptors (TARs) can be activated by tyramine (TA) or octopamine (OA) and have been shown to be related to physiological regulation (e.g., gustatory responsiveness, social organization, and learning behavior) in a range of insect species. A tyramine receptor gene in Plutella xylostella, Pxtar1, was cloned and stably expressed in the HEK-293 cell line. Pharmacological properties and expression profile of Pxtar1 were also analyzed. Tyramine could activate the PxTAR1 receptor, increasing the intracellular Ca²⁺ concentration ((Ca²⁺)i) at an EC50 of 13.1 nM and reducing forskolin (10 μM)-stimulated intracellular cAMP concentration ((cAMP)i) at an IC₅₀ of 446 nM. DPMF (a metabolite of amitraz) and L(-)-carvone (an essential oil) were

SUBMITTER: Ma H 

PROVIDER: S-EPMC6627746 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature