Comparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis.

ABSTRACT: Bordetella pertussis is a Gram-negative strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Previously, we have shown that RNA chaperone Hfq is required for virulence of B. pertussis. Furthermore, microarray analysis revealed that a large number of genes are affected by the lack of Hfq. This study represents the first attempt to characterize the Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expression profiles were analyzed by RNA-seq and protein amounts in cell-associated and cell-free fractions were determined by LC-MS/MS technique. Comparative analysis of transcriptomic and proteomic data revealed solid correlation (r² = 0.4) considering the role of Hfq in post-transcriptional co

SUBMITTER: Dienstbier A 

PROVIDER: S-EPMC6627887 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature