A Rapid and Accurate MinION-Based Workflow for Tracking Species Biodiversity in the Field.

ABSTRACT: Genetic markers (DNA barcodes) are often used to support and confirm species identification. Barcode sequences can be generated in the field using portable systems based on the Oxford Nanopore Technologies (ONT) MinION sequencer. However, to achieve a broader application, current proof-of-principle workflows for on-site barcoding analysis must be standardized to ensure a reliable and robust performance under suboptimal field conditions without increasing costs. Here, we demonstrate the implementation of a new on-site workflow for DNA extraction, PCR-based barcoding, and the generation of consensus sequences. The portable laboratory features inexpensive instruments that can be carried as hand luggage and uses standard molecular biology protocols and reagents that tolerate adverse environmen

SUBMITTER: Maestri S 

PROVIDER: S-EPMC6627956 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature