Engineering CatM, a LysR-Type Transcriptional Regulator, to Respond Synergistically to Two Effectors.

ABSTRACT: The simultaneous response of one transcriptional regulator to different effectors remains largely unexplored. Nevertheless, such interactions can substantially impact gene expression by rapidly integrating cellular signals and by expanding the range of transcriptional responses. In this study, similarities between paralogs were exploited to engineer novel responses in CatM, a regulator that controls benzoate degradation in Acinetobacter baylyi ADP1. One goal was to improve understanding of how its paralog, BenM, activates transcription in response to two compounds (cis,cis-muconate and benzoate) at levels significantly greater than with either alone. Despite the overlapping functions of BenM and CatM, which regulate many of the same ben and cat genes, CatM norma

SUBMITTER: Tumen-Velasquez MP 

PROVIDER: S-EPMC6628147 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature