Ligand-Based Stability Changes in Duplex DNA Measured with a Microscale Electrochemical Platform.

ABSTRACT: Development of technologies for rapid screening of DNA secondary structure thermal stability and the effects on stability for binding of small molecule drugs is important to the drug discovery process. In this report, we describe the capabilities of an electrochemical, microdevice-based approach for determining the melting temperatures (Tm) of electrode-bound duplex DNA structures. We also highlight new features of the technology that are compatible with array development and adaptation for high-throughput screening. As a foundational study to exhibit device performance and capabilities, melting-curve analyses were performed on 12-mer DNA duplexes in the presence/absence of two binding ligands: diminazene aceturate (DMZ) and proflavine. By measuring electrochemical current as a function of

SUBMITTER: Robinson SM 

PROVIDER: S-EPMC6628196 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature