A Multidrug Resistance Plasmid pIMP26, Carrying bla_IMP-26, fosA5, bla_DHA-1, and qnrB4 in Enterobacter cloacae.

ABSTRACT: IMP-26 was a rare IMP variant with more carbapenem-hydrolyzing activities, which was increasingly reported now in China. This study characterized a transferable multidrug resistance plasmid harboring bla_IMP-26 from one Enterobacter cloacae bloodstream isolate in Shanghai and investigated the genetic environment of resistance genes. The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using broth microdilution method, Etest and PCR. The plasmid was analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis and hybridization. Whole genome sequencing and sequence analysis was conducted for further investigation of the plasmid. E. cloacae RJ702, belonging to ST528 and carrying bla_IMP-26, bla_DHA-1

SUBMITTER: Wang S 

PROVIDER: S-EPMC6629617 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature