Environmentally-Controlled Near Infrared Spectroscopic Imaging of Bone Water.

ABSTRACT: We have designed an environmentally-controlled chamber for near infrared spectroscopic imaging (NIRSI) to monitor changes in cortical bone water content, an emerging biomarker related to bone quality assessment. The chamber is required to ensure repeatable spectroscopic measurements of tissues without the influence of atmospheric moisture. A calibration curve to predict gravimetric water content from human cadaveric cortical bone was created using NIRSI data obtained at six different lyophilization time points. Partial least squares (PLS) models successfully predicted bone water content that ranged from 0-10% (R = 0.96, p < 0.05, root mean square error of prediction (RMSEP) = 7.39%), as well as in the physiologic range of 4-10% of wet tissue weight (R = 0.87, p < 0.05, RMSEP = 14.5%). Simi

SUBMITTER: Ailavajhala R 

PROVIDER: S-EPMC6629628 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature