Immunocyte Profiling Using Single-Cell Mass Cytometry Reveals EpCAM⁺ CD4⁺ T Cells Abnormal in Colon Cancer.

ABSTRACT: Colon cancer (CC) is one of the leading causes of cancer related mortality. Research over past decades have profoundly enhanced our understanding of immunotherapy, a major clinical accomplishment, and its potential role toward treating CC. However, studies investigating the expression of these immune checkpoints, such as epithelial cell adhesion molecule (EpCAM), programmed death-1 (PD-1), and programmed death-ligand 1 (PD-L1), by peripheral blood mononuclear cells (PBMCs) is lacking. Here, high-dimensional mass cytometry (CyTOF) is used to investigate immune alterations and promising immunotherapeutic targets expression by PBMCs of CC patients. Results reveal that expression of EpCAM and PD-L1 on CD4⁺ T cells significantly increased in patients with CC, compared with age- and s

SUBMITTER: Zhang T 

PROVIDER: S-EPMC6629930 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature