Long noncoding RNA ENST00000413528 sponges microRNA-593-5p to modulate human glioma growth via polo-like kinase 1.

ABSTRACT:

Aims

In this study, we examined the expression of lncRNA ENST00000413528 in glioma and determined its role in glioma development.

Methods

LncRNA ENST00000413528 was detected in glioma tissues by lncRNA microarray. Then, we performed real-time PCR, CCK-8, colony formation assay, flow cytometry, caspase-3/7 assay and animal experiment to detect the function of ENST00000413528 in glioma after ENST00000413528 knockdown. Subsequent bioinformatics analysis, luciferase reporter assays and RNA immunoprecipitation (RIP) assay western blotting indicated possible downstream regulatory molecules. The expression of PLK1 in glioma tissues was also examined by immunohistochemistry staining.

Results

Expression of ENST00000413528 was significantly increased in glioma tissues and LN229

SUBMITTER: Zhang R 

PROVIDER: S-EPMC6630009 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature