Multimodal Tracking of Controlled Staphylococcus aureus Infections in Mice.

ABSTRACT: There is a need to develop diagnostic and analytical tools that allow noninvasive monitoring of bacterial growth and dissemination in vivo. For such cell-tracking studies to hold translational value to controlled human infections, in which volunteers are experimentally colonized, they should not require genetic modification, and they should allow tracking over a number of replication cycles. To gauge if an antimicrobial peptide tracer, ^99mTc-UBI_29-41-Cy5, which contains both a fluorescent and a radioactive moiety, could be used for such in vivo bacterial tracking, we performed longitudinal imaging of a thigh-muscle infection with ^99mTc-UBI_29-41-Cy5-labeled Staphylococcus aureus. Mice were imaged using SPECT and fluorescence-imaging modalit

SUBMITTER: Welling MM 

PROVIDER: S-EPMC6630532 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature