Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay.

ABSTRACT:

Objective

Aconitum plants (Ranunculaceae) exhibit toxicity, and accidental ingestion of the plants has been reported in Japan. Identifying the cause of poisoning is important for emergency medical treatment, and a rapid and simple detection technique is required for the identification of poisoning cause. In the present study, we developed a rapid and simple method for detecting Aconitum plant DNA using a loop-mediated isothermal amplification (LAMP) assay.

Results

Specific LAMP primers for Aconitum plants were designed based on the trnL-trnF intergenic spacer region. Using the LAMP primers, the LAMP assay included an initiation reaction of 10 min followed by amplification for 20 min at the isothermal reaction temperature of 65 °C. The LAMP reaction was demonstrated to be spe

SUBMITTER: Kitamura M 

PROVIDER: S-EPMC6631447 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature