RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection.

ABSTRACT: Chikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards Aedesalbopictus. While mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. We performed whole transcriptome analysis on dissected midguts of Aedes albopictus infected with CHIKV to identify differentially expressed genes. For this, RNA was extracted at two days post-infection (2-dpi) from pooled midguts. We initially identified 25 differentially expressed genes (p-value < 0.05) when mapped to a reference transcriptom

SUBMITTER: Vedururu RK 

PROVIDER: S-EPMC6631752 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature