Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus.

ABSTRACT: In the biopharmaceutical pipeline, protein expression systems are of high importance not only for the production of biotherapeutics but also for the discovery of novel drugs. The vast majority of drug targets are proteins, which need to be characterized and validated prior to the screening of potential hit components and molecules. A broad range of protein expression systems is currently available, mostly based on cellular organisms of prokaryotic and eukaryotic origin. Prokaryotic cell-free systems are often the system of choice for drug target protein production due to the simple generation of expression hosts and low cost of preparation. Limitations in the production of complex mammalian proteins appear due to inefficient protein folding and posttranslational modifications. Alternative

SUBMITTER: Thoring L 

PROVIDER: S-EPMC6632147 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature