P53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition.

ABSTRACT:

Background

We reported previously that phenethyl isothiocyanate (PEITC), a dietary compound, can reactivate p53^R175H mutant in vitro and in SK-BR-3 (p53^R175H) breast xenograft model resulting in tumor inhibition. Because of the diversity of human cancers with p53 mutations, these findings raise important questions whether this mechanism operates in different cancer types with same or different p53 mutations. In this study, we investigated whether PEITC recuses mutant p53 in prostate cancer cells harboring different types of p53 mutants, structural and contact, in vitro and in vivo.

Methods

Cell proliferation, cell apoptosis and cell cycle arrest assays were performed to examine the effects of PEITC on prostate cancer cell lines with p53 mutation(s), wil

SUBMITTER: Aggarwal M 

PROVIDER: S-EPMC6632191 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature