A highly ordered, nonprotective MALAT1 ENE structure is adopted prior to triplex formation.

ABSTRACT: The 3' end of the ∼7 kb lncRNA MALAT1 contains an evolutionarily and structurally conserved element for nuclear expression (ENE) which confers protection from cellular degradation pathways. Formation of an ENE triple helix is required to support transcript accumulation, leading to persistent oncogenic activity of MALAT1 in multiple cancer types. Though the specific mechanism of triplex-mediated protection remains unknown, the MALAT1 ENE triplex has been identified as a promising target for therapeutic intervention. Interestingly, a maturation step of the nascent lncRNA 3' end is required prior to triplex formation. We hypothesize that disruption of the maturation or folding process may be a viable mechanism of inhibition. To assess putative cotranscriptional ENE conformations prior to trip

SUBMITTER: Yonkunas MJ 

PROVIDER: S-EPMC6633196 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature