Phenotype of ribonuclease 1 deficiency in mice.

ABSTRACT: Biological roles for extracellular RNA (eRNA) have become apparent. For example, eRNA can induce contact activation in blood via activation of the plasma proteases factor XII (FXII) and factor XI (FXI). We sought to reveal the biological role of the secretory enzyme ribonuclease 1 (RNase 1) in an organismal context by generating and analyzing RNase 1 knockout (Rnase1 ^-/-) mice. We found that these mice are viable, healthy, and fertile, though larger than Rnase1 ^+/+ mice. Rnase1 ^-/- plasma contains more RNA than does the plasma of Rnase1 ^+/+ mice. Moreover, the plasma of Rnase1 ^-/- mice clots more rapidly than does wild-type plasma. This phenotype appeared to be due to increased levels of the active fo

SUBMITTER: Garnett ER 

PROVIDER: S-EPMC6633200 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature