Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues.

ABSTRACT: Visualization of gene expression at single RNA molecular level represents a great challenge to both imaging technologies and molecular engineering. Here we show a single molecule chromogenic in situ hybridization (smCISH) assay that enables counting and localizing individual RNA molecules in fixed cells and tissue under bright-field microscopy. Our method is based on in situ padlock probe assays directly using RNA as a ligation template and rolling circle amplification combined with enzyme catalyzed chromogenic reaction for amplification product visualization. We show potential applications of our method by detecting gene expression variations in single cells, subcellular localization information of expressed genes, and gene expression heterogeneity in formalin-fixed, paraffin-embedded tis

SUBMITTER: Jiang M 

PROVIDER: S-EPMC6633204 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature