Early genome activation in Drosophila is extensive with an initial tendency for aborted transcripts and retained introns.

ABSTRACT: Control of metazoan embryogenesis shifts from maternal to zygotic gene products as the zygotic genome becomes transcriptionally activated. In Drosophila, zygotic genome activation (ZGA) has been thought to occur in two phases, starting with a minor wave, in which a small number of genes become expressed, and progressing to the major wave, in which many more genes are activated. However, technical challenges have hampered the identification of early transcripts or obscured the onset of their transcription. Here, we develop an approach to isolate transcribed mRNAs and apply it over the course of Drosophila early genome activation. Our results increase by 10-fold the genes reported to be activated during what has been thought of as the minor wave and show that early genome activ

SUBMITTER: Kwasnieski JC 

PROVIDER: S-EPMC6633261 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature