Dual control of RegX3 transcriptional activity by SenX3 and PknB.

ABSTRACT: The mycobacterial SenX3-RegX3 two-component system consists of the SenX3 sensor histidine kinase and its cognate RegX3 response regulator. This system is a phosphorelay-based regulatory system involved in sensing environmental P_i levels and induction of genes required for P_i acquisition under P_i-limiting conditions. Here we demonstrate that overexpression of the kinase domain of Mycobacterium tuberculosis PknB (PknB-KD^Mtb) inhibits the transcriptional activity of RegX3 of both M. tuberculosis and Mycobacterium smegmatis (RegX3^Mtb and RegX3^Ms, respectively). Mass spectrometry results, along with those of in vitro phosphorylation and complementation analyses, revealed that PknB kinase activity inhib

SUBMITTER: Park EJ 

PROVIDER: S-EPMC6635444 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature