Label-free time- and space-resolved exometabolite sampling of growing plant roots through nanoporous interfaces.

ABSTRACT: Spatial and temporal profiling of metabolites within and between living systems is vital to understanding how chemical signaling shapes the composition and function of these complex systems. Measurement of metabolites is challenging because they are often not amenable to extrinsic tags, are diverse in nature, and are present with a broad range of concentrations. Moreover, direct imaging by chemically informative tools can significantly compromise viability of the system of interest or lack adequate resolution. Here, we present a nano-enabled and label-free imaging technology using a microfluidic sampling network to track production and distribution of chemical information in the microenvironment of a living organism. We describe the integration of a polyester track-etched (PETE) nanofluidi

SUBMITTER: Patabadige DEW 

PROVIDER: S-EPMC6635491 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature