Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation.

ABSTRACT: Previous studies have shown that T cell receptor (TCR) and CD28 coreceptor stimulation involves rapid ATP release, autocrine purinergic feedback via P2X receptors, and mitochondrial ATP synthesis that promote T cell activation. Here, we show that ADP formation and autocrine stimulation of P2Y1 receptors are also involved in these purinergic signaling mechanisms. Primary human CD4 T cells and the human Jurkat CD4 T cell line express P2Y1 receptors. The expression of this receptor increases following T cell stimulation. Inhibition of P2Y1 receptors impairs the activation of mitochondria, as assessed by mitochondrial Ca²⁺ uptake, and reduces cytosolic Ca²⁺ signaling in response to TCR/CD28 stimulation. We found that the addition of exogenous ADP or overexpression of P2Y1

SUBMITTER: Woehrle T 

PROVIDER: S-EPMC6635541 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature