Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+ and CD8+ T Cells.

ABSTRACT: While genetic traits and epigenetic modifications mainly encode cell type-specific effector functions, the eventual outcome is also prone to modulation by post-transcriptional regulation mechanisms. T cells are a powerful model for the investigation of such modulatory effects, as common precursor cells may differentiate either to helper CD4⁺ T cells or cytotoxic CD8⁺ cells, which elicit distinct functionalities upon TCR-stimulation. Human primary CD4⁺ and CD8⁺ T cells were purified from three individual donors and activated with anti-CD3/CD28 antibodies. Associated proteome alterations were analyzed by high-resolution mass spectrometry using a label-free shotgun approach. Metabolic activation was indicated by upregulation of enzymes related to gl

SUBMITTER: Gerner MC 

PROVIDER: S-EPMC6635586 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature