Genomic DNA methylation distinguishes subtypes of human focal cortical dysplasia.

ABSTRACT:

Objectives

Focal cortical dysplasia (FCD) is a major cause of drug-resistant focal epilepsy in children, and the clinicopathological classification remains a challenging issue in daily practice. With the recent progress in DNA methylation-based classification of human brain tumors we examined whether genomic DNA methylation and gene expression analysis can be used to also distinguish human FCD subtypes.

Methods

DNA methylomes and transcriptomes were generated from massive parallel sequencing in 15 surgical FCD specimens, matched with 5 epilepsy and 6 nonepilepsy controls.

Results

Differential hierarchical cluster analysis of DNA methylation distinguished major FCD subtypes (ie, Ia, IIa, and IIb) from patients with temporal lobe epilepsy patients and nonepileptic contr

SUBMITTER: Kobow K 

PROVIDER: S-EPMC6635741 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature