LncRNA-mRNA expression profiles and functional networks of mesenchymal stromal cells involved in monocyte regulation.

ABSTRACT:

Background

The goals of this study were to explore the expression profiles and functional networks of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in mesenchymal stromal cells (MSCs) involved in regulating the function of monocytes and to clarify the mechanisms by which MSCs exert immunoregulatory effects on monocytes.

Methods

MSCs and CD14+ monocytes were separately isolated. The immunoregulatory effects of MSCs on monocytes were determined by flow cytometry. lncRNAs and mRNAs that were differentially expressed (DE) between the control group (MSCs only) and co-culture group (MSCs co-cultured with monocytes) were identified through high-throughput sequencing and bioinformatic analyses and were confirmed by qRT-PCR. Bioinformatic analyses were performed to identi

SUBMITTER: Li M 

PROVIDER: S-EPMC6636070 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature