Heterologous Ectoine Production in Escherichia coli: Optimization Using Response Surface Methodology.

ABSTRACT:

Introduction

A halophilic bacterium of the Halomonas elongata BK-AG25 has successfully produced ectoine with high productivity. To overcome the drawbacks of high levels of salt in the production process, a nonhalophilic bacteria of Escherichia coli (E. coli) was used to express the ectoine gene cluster of the halophilic bacteria, and the production of ectoine by the recombinant cell was optimized.

Methods

The ectoine gene cluster from the halophilic bacterium was isolated and inserted into an expression plasmid of pET30(a) and subsequently transformed into E. coli BL21 (DE3). Production of ectoine from the recombinant E. coli was investigated and then maximized by optimizing the level of nutrients in the medium, as well as the bioprocess co

SUBMITTER: Parwata IP 

PROVIDER: S-EPMC6636453 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature