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Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae).

ABSTRACT:

Premise

Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus.

Methods and results

Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in L. aspera, L. microcephala, and L. pycnostachya.

Conclusions

The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.

SUBMITTER: Clark LC

PROVIDER: S-EPMC6636620 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Development of 17 microsatellite markers in the federally endangered species <i>Liatris helleri</i> (Asteraceae).

Clark Logan C LC Gaglianese-Woody Morgan R MR Estep Matt C MC

Applications in plant sciences 20190517 7

<h4>Premise</h4>Microsatellite markers were developed in the federally endangered species <i>Liatris helleri</i> (Asteraceae) to evaluate species boundaries with closely related congeners within the genus.<h4>Methods and results</h4>Using Illumina data, 17 primer pairs were developed in populations of <i>L. helleri</i>. The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in <i>L. aspera</i>, <i>L. mic ...[more]

PMID: 31346504

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