Partial enzymatic reactions: A missed opportunity in proteomics research.

ABSTRACT:

Rationale

Biological studies are conducted at ever-increasing rates by relying on proteomic workflows. Although data acquisition by mass spectrometry is highly automated and rapid, sample preparation continues to be the bottleneck of developing high-throughput workflows. Enzymatic protein processing, in particular, involves time-consuming protocols that can extend from one day to another. To address this gap, we developed and evaluated simple, in-solution tryptic enzymatic reactions that unfold within a few minutes, and demonstrate the utility of the methodology for the rapid analysis of proteins originating from cancer cell extracts.

Methods

Tryptic enzymatic reactions were conducted for 7-60 min, and the results were compared with that of a routine approach conducted for 1

SUBMITTER: Deng J 

PROVIDER: S-EPMC6636927 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature