Ca²⁺ signalling plays a role in celastrol-mediated suppression of synovial fibroblasts of rheumatoid arthritis patients and experimental arthritis in rats.

ABSTRACT:

Background and purpose

Celastrol exhibits anti-arthritic effects in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca²⁺ mobilization in treatment of RA remains undefined. Here, we describe a regulatory role for celastrol-induced Ca²⁺ signalling in synovial fibroblasts of RA patients and adjuvant-induced arthritis (AIA) in rats.

Experimental approach

We used computational docking, Ca²⁺ dynamics and functional assays to study the sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase pump (SERCA). In rheumatoid arthritis synovial fibroblasts (RASFs)/rheumatoid arthritis fibroblast-like synoviocytes (RAFLS), mechanisms of Ca²⁺ -mediated autophagy were analysed by histological, immunohistochemical and flow cytometric

SUBMITTER: Wong VKW 

PROVIDER: S-EPMC6637043 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature