Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm.

ABSTRACT: Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout t

SUBMITTER: Kim M 

PROVIDER: S-EPMC6637127 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature