Proteogenomic Analysis of Protein Sequence Alterations in Breast Cancer Cells.

ABSTRACT: Cancer evolves as a result of an accumulation of mutations and chromosomal aberrations. Developments in sequencing technologies have enabled the discovery and cataloguing of millions of such mutations. The identification of protein-level alterations, typically by using reversed-phase protein arrays or mass spectrometry, has lagged, however, behind gene and transcript-level observations. In this study, we report the use of mass spectrometry for detecting the presence of mutations-missense, indels and frame shifts-in MCF7 and SKBR3 breast cancer, and non-tumorigenic MCF10A cells. The mutations were identified by expanding the database search process of raw mass spectrometry files by including an in-house built database of mutated peptides (XMAn-v1) that complemented a minimally redundant, ca

SUBMITTER: Lazar IM 

PROVIDER: S-EPMC6637242 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature