Circular RNA expression profiles and bioinformatics analysis in ovarian endometriosis.

ABSTRACT:

Background

Circular RNAs (circRNAs) with miRNA response elements (MREs) could function as competing endogenous RNA (ceRNA) in regulating gene expression, thus playing vital roles in pathogenesis and progression of many diseases. However, the function of circRNAs in endometriosis remains unknown. This study was carried to profile the expression patterns of circRNAs in ovarian endometriosis.

Methods

High throughput RNA-Seq was performed in six paired ectopic and eutopic endometrium tissues (ecEM vs. euEM), followed by quantitative real-time polymerase chain reaction (qRT-PCR) in 30 paired samples. Through bioinformatics prediction, we constructed a circRNA-miRNA -mRNA network and elucidated circRNAs functions by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (K

SUBMITTER: Wang D 

PROVIDER: S-EPMC6637292 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature