Crosslinked flagella as a stabilized vaccine adjuvant scaffold.

ABSTRACT:

Background

Engineered vaccine proteins incorporating both antigen and adjuvant components are constructed with the aim of combining functions to induce effective protective immunity. Bacterial flagellin is a strong candidate for an engineered vaccine scaffold as it is known to provide adjuvant activity through its TLR5 and inflammasome activation. Moreover, polymerized flagellin filaments can elicit a more robust immunoglobulin response than monomeric flagellin, and the multimeric antigen form can also promote T cell-independent antibody responses. Here, we aim to produce and test a covalently stabilized polymerized flagellar filament, providing additional immune efficacy through stabilization of its polymeric filament structure, as well as stabilization for long-term storage.

R

SUBMITTER: Gries CM 

PROVIDER: S-EPMC6637544 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature