De novo transcriptome sequencing and gene expression profiling of Magnolia wufengensis in response to cold stress.

ABSTRACT:

Background

Magnolia wufengensis is a new species of Magnolia L. and has considerable ornamental and economic value due to its unique characteristics. However, because of its characteristic of poor low temperature resistance, M. wufengensis is hardly popularization and application in the north of China. Furthermore, the mechanisms of gene regulation and signaling pathways involved in the cold-stress response remained unclear in this species. In order to solve the above-mentioned problems, we performed de novo transcriptome assembly and compared the gene expression under the natural (25 °C) and cold (4 °C) conditions for M. wufengensis seedlings.

Results

More than 46 million high-quality clean reads were produced from six samples (RNA was extracted from the leaves) and were us

SUBMITTER: Deng S 

PROVIDER: S-EPMC6637634 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature