A Na⁺ /Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence.

ABSTRACT: In a number of compatible plant-bacterium interactions, a rise in apoplastic Ca²⁺ levels is observed, suggesting that Ca²⁺ represents an important environmental clue, as reported for bacteria infecting mammalians. We demonstrate that Ca²⁺ entry in Pseudomonas savastanoi pv. savastanoi (Psav) strain DAPP-PG 722 is mediated by a Na⁺ /Ca²⁺ exchanger critical for virulence. Using the fluorescent Ca²⁺ probe Fura 2-AM, we demonstrate that Ca²⁺ enters Psav cells foremost when they experience low levels of energy, a situation mimicking the apoplastic fluid. In fact, Ca²⁺ entry was suppressed in the presence of high concentrations of glucose, fructose, sucrose or adenosine triphosphate (ATP). Since Ca²⁺

SUBMITTER: Moretti C 

PROVIDER: S-EPMC6637891 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature