Identification by Tn-seq of Dickeya dadantii genes required for survival in chicory plants.

ABSTRACT: The identification of the virulence factors of plant-pathogenic bacteria has relied on the testing of individual mutants on plants, a time-consuming process. Transposon sequencing (Tn-seq) is a very powerful method for the identification of the genes required for bacterial growth in their host. We used this method in a soft-rot pathogenic bacterium to identify the genes required for the multiplication of Dickeya dadantii in chicory. About 100 genes were identified showing decreased or increased fitness in the plant. Most had no previously attributed role in plant-bacterium interactions. Following our screening, in planta competition assays confirmed that the uridine monophosphate biosynthesis pathway and the purine biosynthesis pathway were essential to the survival of D. dadantii in the p

SUBMITTER: Royet K 

PROVIDER: S-EPMC6637903 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature