Response regulator VemR regulates the transcription of flagellar rod gene flgG by interacting with σ⁵⁴ factor RpoN2 in Xanthomonas citri ssp. citri.

ABSTRACT: Xanthomonas citri ssp. citri, a polar flagellated bacterium, causes citrus canker disease worldwide. In this study, we found that the X. citri ssp. citri response regulator VemR plays a regulatory role in flagellum-derived cell motility. Deletion of the vemR gene resulted in a reduction in cell motility, as well as reductions in virulence and exopolysaccharide production. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that vemR is transcribed in an operon together with rpoN2 and fleQ. In the vemR mutant, the flagellar distal rod gene flgG was significantly down-regulated. Because flgG is also rpoN2 dependent, we speculated that VemR and RpoN2 physically interact, which was confirmed by yeast two-hybrid and maltose-binding protein (MBP) pull-down assays. This suggeste

SUBMITTER: Wu W 

PROVIDER: S-EPMC6637908 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature