Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China.

ABSTRACT:

Background

Distinguishing among species in the genus Lepista is difficult because of their similar morphologies.

Methods

To identify a suitable DNA barcode for identification of Lepista species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight Lepista species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.

Results

The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS

SUBMITTER: Wang S 

PROVIDER: S-EPMC6637932 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature