Ontology highlight
ABSTRACT:
SUBMITTER: Shen H
PROVIDER: S-EPMC6637945 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20181101 6415
We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo-electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the numb ...[more]