Truncation of a P1 leader proteinase facilitates potyvirus replication in a non-permissive host.

ABSTRACT: The Potyviridae family is a major group of plant viruses that includes c. 200 species, most of which have narrow host ranges. The potyvirid P1 leader proteinase self-cleaves from the remainder of the viral polyprotein and shows large sequence variability linked to host adaptation. P1 proteins can be classified as Type A or Type B on the basis, amongst other things, of their dependence or not on a host factor to develop their protease activity. In this work, we studied Type A proteases from the Potyviridae family, characterizing their host factor requirements. Our in vitro cleavage analyses of potyvirid P1 proteases showed that the N-terminal domain is relevant for host factor interaction and suggested that the C-terminal domain is also involved. In the absence of plant factors, the N-termi

SUBMITTER: Shan H 

PROVIDER: S-EPMC6638051 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature