A modular toolbox for Golden-Gate-based plasmid assembly streamlines the generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.

ABSTRACT: Members of the Ralstonia solanacearum species complex (Rssc) cause bacterial wilt, a devastating plant disease that affects numerous economically important crops. Like other bacterial pests, Rssc injects a cocktail of effector proteins via the bacterial type III secretion system into host cells that collectively promote disease. Given their functional relevance in disease, the identification of Rssc effectors and the investigation of their in planta function are likely to provide clues on how to generate pest-resistant crop plants. Accordingly, molecular analysis of effector function is a focus of Rssc research. The elucidation of effector function requires corresponding gene knockout strains or strains that express the desired effector variants. The cloning of DNA constructs that facilita

SUBMITTER: Wu D 

PROVIDER: S-EPMC6638168 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature