Characterization of two catalase-peroxidase-encoding genes in Fusarium verticillioides reveals differential responses to in vitro versus in planta oxidative challenges.

ABSTRACT: Catalase-peroxidases (KatGs) are a superfamily of reactive oxygen species (ROS)-degrading enzymes believed to have been horizontally acquired by ancient Ascomycota from bacteria. Subsequent gene duplication resulted in two KatG paralogues in ascomycetes: the widely distributed intracellular KatG1 group and the phytopathogen-dominated extracellular KatG2 group. To functionally characterize FvCP01 (KatG1) and FvCP02 (KatG2) in the maize pathogen Fusarium verticillioides, single and double gene deletion mutants were examined in response to hydrogen peroxide (H₂ O₂ ). Both ΔFvCP01 and ΔFvCP02 were more sensitive to H₂ O₂ than the wild-type in vitro, although their sensitivity differed depending on the type of inoculum. Inoculations using mycelial aga

SUBMITTER: Gao S 

PROVIDER: S-EPMC6638182 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature