Production of small cysteine-rich effector proteins in Escherichia coli for structural and functional studies.

ABSTRACT: Although the lifestyles and infection strategies of plant pathogens are diverse, a prevailing feature is the use of an arsenal of secreted proteins, known as effectors, which aid in microbial infection. In the case of eukaryotic filamentous pathogens, such as fungi and oomycetes, effector proteins are typically dissimilar, at the protein sequence level, to known protein families and functional domains. Consequently, we currently have a limited understanding of how fungal and oomycete effectors promote disease. Protein biochemistry and structural biology are two methods that can contribute greatly to the understanding of protein function. Both techniques are dependent on obtaining proteins that are pure and functional, and generally require the use of heterologous recombinant protein expres

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6638209 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature