An engineered promoter driving expression of a microbial avirulence gene confers recognition of TAL effectors and reduces growth of diverse Xanthomonas strains in citrus.

ABSTRACT: Xanthomonas citri ssp. citri (X. citri), causal agent of citrus canker, uses transcription activator-like effectors (TALEs) as major pathogenicity factors. TALEs, which are delivered into plant cells through the type III secretion system (T3SS), interact with effector binding elements (EBEs) in host genomes to activate the expression of downstream susceptibility genes to promote disease. Predictably, TALEs bind EBEs in host promoters via known combinations of TALE amino acids to DNA bases, known as the TALE code. We introduced 14 EBEs, matching distinct X. citri TALEs, into the promoter of the pepper Bs3 gene (ProBs3_1EBE ), and fused this engineered promoter with multiple EBEs (ProBs3_14EBE ) to either the β-glucuronidase (GUS) reporter gene or the coding sequence (cds

SUBMITTER: Shantharaj D 

PROVIDER: S-EPMC6638256 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature